Assessment of antibacterial and antioxidant activity of aqueous crude flower, leaf, and bark extracts of Ethiopian <i>Hibiscus rosa-sinensis</i> Linn: geographical effects and Co₂Res₂ /Glassy carbon electrode

Ethiopian Hibiscus rosa-sinensis Linn contains a variety of bioactive phytochemicals, which possess several biological activities. This study aims to analyze the antibacterial and antioxidant activity aqueous crude flower, leaf, bark extracts rosa-sinensis, as well see geographical effects method development Co2Res2/Glassy carbon electrode. The secondary metabolites were investigated by maceration techniques. Disc diffusion methods are used for antimicrobial activities against total four human pathogens bacteria such E. coli, K. pneumonia, S. aureus, epidermidis, respectively. Antioxidant assessed using differential pulse voltammetry (DPV), whereas, phytoconstituents profiled FTIR UV-Vis analysis. phytochemical investigations showed that phenols, flavonoids, quinines observed more exist in whereas flavonoids moderately but steroids, terpenoids, cardiac glycosides have not existed leaf plant growth inhibitory effect aureus epidermidis range 6.33 ± 0.33 11.50 0.29 mm, was recorded pneumonia coli 6.67 13.00 .58 XRD analysis confirmed Co(II) based MOF, Co2Res2 nanosize structure with an average crystal size 27.17 nm. Furthermore, DPV net modified current peaks potentials most significant compared pure potential fresh extracted from rosa-sinensis. These shown improved source natural antioxidants Chemically thermally stable porous crystalline serves catalytic reactions increase decrease GCE, this might be due synergetic cumulative presence ingredients catalyst.Abbreviations: ABS: Acetate buffer solution; ANOVA: Analysis variance; ATR: Attenuated reflectance; CFU/ml: Colony forming unit per milliliter: Co2Res2: metal-organic framework (ResH2: resorcinol); DPV: Differential plus voltammetry: FTIR; Fourier transforms infrared radiation; GCE: Glassy electrode; JCPDS: A joint committee on power diffraction standards; MOF: Metal-organic framework; PBS: Phosphate SPSS: Statistical package social sciences; UV-Vis: Ultraviolet-visible spectroscopy; XRD: X-ray